Journal: Virology Journal

Article Title: Comparison of the detection performance of two different one-step-combined test strips with fluorescent microspheres or colored microspheres as tracers for influenza A and B viruses

doi: 10.1186/s12985-019-1190-0

Figure Lengend Snippet: Test modes of the two combined detection test strips . a Colored microspheres for tracers (CMT): Control line (blue); Detection line (red) including the influenza A line (near the sample loading end) and the influenza B line. Interpretation of results: after the test procedure, negative (left) and double positive (right) results were detected. b Fluorescent microspheres for tracers (FMT): Control line; Detection line including the influenza A line (near the sample loading well) and the influenza B line. Interpretation of results: after the test procedure, negative (left) and double positive (right) results were detected

Article Snippet: The tracers were fluorescent microspheres (phenylethene luminative monomeric copolymers; approximate diameter 200 nm) with excitation and emission wavelengths of 360 nm and 615 nm, respectively (Suzhou Vdo Biotech Co., Ltd., Suzhou, China).

Techniques: Control

Journal: Scientific reports

Article Title: Multiline orthogonal scanning temporal focusing (mosTF) microscopy for scattering reduction in in vivo brain imaging.

doi: 10.1038/s41598-024-57208-6

Figure Lengend Snippet: Figure 1. Schematic of mosTF imaging system. (a) mosTF setup. Flip mirrors (M1-M3) are utilized to direct light along two alternative paths, thereby rotating the scanning orientation (indicated by the dashed box). Refer to the Methods section for detailed information about the setup. (b–e) mosTF reconstruction process. (b) The object to be imaged through scattering media. (c) mosTF captures each intermediate image at every scan position. The dashed lines indicate the locations of the four scanning lines. (d) The algorithm then sums scattered photons within the adjacent area (rs) back to the scanning line position. This process is repeated for all n intermediate images collected during scanning in both the vertical and horizontal directions. (e) The combination of reconstructed images from both directions results in the final image. (f) Measured PSF of mosTF (left) and the corresponding lateral and axial intensity profiles of the PSF (right top and bottom, respectively). Fluorescent nanoparticles of 200 nm size were used for this measurement. Scale bar, 1 µm.

Article Snippet: Our samples consisted of 200 nm diameter red fluorescent beads (the same as used for the PSF measurement) mounted in clear medium (Fluoromount-G®, SouthernBiotech, AL, USA).

Techniques: Imaging

Journal: Scientific Reports

Article Title: Transmission structured illumination microscopy with tunable frequency illumination using tilt mirror assembly

doi: 10.1038/s41598-023-27814-x

Figure Lengend Snippet: Experimental results of fluorescent beads: ( a ) single FOV tSIM reconstruction and widefield image of 200 nm fluorescent beads; ( b ) diffraction limited spectra; ( c ) reconstructed tSIM spectra; ( d , f ) magnified widefield images of white box in R \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$_1$$\end{document} 1 and R \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$_2$$\end{document} 2 regions, respectively; ( e , g ) closeup of the tSIM reconstruction in R \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$_1$$\end{document} 1 and R \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$_2$$\end{document} 2 regions, respectively; ( h ) intensity line-profiles of ( d , e ); ( i ) intensity line-profiles of ( f , g ).

Article Snippet: A small (0.5 μl) amount of 200-nm diameter fluorescent bead solution (Suncoast yellow, FSSY002 Banglabs) was pipetted out and poured gently onto the cleaned coverslip.

Techniques:

Journal: Scientific Reports

Article Title: Transmission structured illumination microscopy with tunable frequency illumination using tilt mirror assembly

doi: 10.1038/s41598-023-27814-x

Figure Lengend Snippet: Simulation result of high-frequency tSIM imaging using synthetic fluorescent particles: ( a ) single FOV tSIM reconstructed image and widefield image using 20X/0.65 detection objective lens and inset shows spectra of the reconstructed image; ( b , f ) ground-truth object of R \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$_1$$\end{document} 1 and R \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$_2$$\end{document} 2 regions; ( c , g ) widefield image of R \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$_1$$\end{document} 1 and R \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$_2$$\end{document} 2 regions; ( d , h ) reconstructed image of R \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$_1$$\end{document} 1 and R \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$_2$$\end{document} 2 regions; ( e , i ) intensity line profiles within the yellow box.

Article Snippet: A small (0.5 μl) amount of 200-nm diameter fluorescent bead solution (Suncoast yellow, FSSY002 Banglabs) was pipetted out and poured gently onto the cleaned coverslip.

Techniques: Imaging

Journal: Neural Regeneration Research

Article Title: BMPRII + neural precursor cells isolated and characterized from organotypic neurospheres: an in vitro model of human fetal spinal cord development

doi: 10.4103/1673-5374.373669

Figure Lengend Snippet: Characterization of fluorescence-assisted cell sorting-sorted BMPRII + hNPCs. (A–E) Sorted cells were plated, fixed, and subjected to immunofluorescence staining with antibodies to O4 (blue), GFAP (red), MAP2ab (green), and the nuclei counterstained with DAPI (magenta). Image (A) shows sorted cells at low magnification. Images in C through E are shown with polarized bright field and merged at a higher magnification in B (from the area of the white box in A). (F) A representative single-label FACS histogram plot of fluorescence intensity versus cell number for collected BMPRII + cells (green trace) and control (2° antibody only; red trace). “Collected” shows the fluorescence intensity gate used to collect the BMPRII + cells. (G) Sorted cells were plated and the proportion of each phenotype was quantified. A significant increase was found for the number of MAP2ab + cells from approximately 24% in control to 83% in collected. (H) Cells were characterized using antibodies against each indicated marker where ‘+’ indicates > 90% of the cells with neuronal morphology were positive and ‘–’ indicates < 10% of these cells were positive. Data are expressed as the mean ± SEM, and scale bars are as indicated. BMP: Bone morphogenetic protein; BMPRII: type-II BMP receptor; DAPI: 4′,6-diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; hNPCs: human neural precursor cells; MAP: microtubule-associated protein; NeuN: neuron-specific nuclear protein; PSA-NCAM: polysialylated-neural cell adhesion molecule.

Article Snippet: Reaggregates were cultured for 21 days in vitro and free-floating spheres between 200–500 μm in diameter were collected and gently triturated in fluorescence-assisted cell sorting (FACS) buffer (3% BSA was added to 10 mM EDTA in NB media without phenol red, Gibco) to dissociate surface cells.

Techniques: Fluorescence, FACS, Immunofluorescence, Staining, Marker